Method Description Sheet

Method EPA Method 547
Title Analysis of Glyphosate in drinking water and source water.
Abstract The water sample is filtered and a 400-uL aliquot is injected into a reverse phase HPLC column. Separation of the analytes is achieved using gradient elution chromatography. After elution from the HPLC column, the analytes are hydrolyzed with 0.05 N sodium hydroxide (NAOH) at 95'C. The methyl amine formed during hydrolysis is reacted with o-phthalaldehyde (OPA) and 2-mercaptoethanol to form a highly fluorescing derivative which is quantified by a fluorescence detector.

Compounds Analyzed :

CAS #Compound Name
1071-83-6 Glyphosate
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