Method Description Sheet

Method EPA Method 555
Title DETERMINATION OF CHLORINATED ACIDS IN WATER BY HIGHPERFORMANCE LIQUID CHROMATOGRAPHY WITH A PHOTODIODE ARRAYULTRAVIOLET DETECTOR
Abstract A measured sample volume of approximately 100 mL is adjusted to pH 12 with 6 N sodium hydroxide, shaken, and allowed to set for 1 hr to hydrolyze chlorinated esters. The sample is acidified with H3PO4, filtered, and the chlorinated acids are extracted from a 25 ml aliquot. The 20-mL aliquot is pumped through an HPLC cartridge (containing C18 -silica) trapping the chlorinated acids. The concentrator cartridge is valved in-line with the Cl8 analytical column following extraction. The analytes are separated and measured by photodiode array-ultraviolet detection (PDA-UV). NOTE: A liquid-solid extraction disk is perfectly acceptable for use in the in-line extraction of the analytes providing all quality control (QC) criteria are met or exceeded.

Compounds Analyzed :

CAS #Compound Name
50594666 Acifluorfen
25057890 Bentazon
133904 Chlorambena
94757 2,4-D
94826 2,4-DB
1918009 Dicamba
51365 3,5-Dichlorobenzoic acid
120365 Dichlorprop
88857 Dinoseb
7600502 5-Hydroxydicamba
94746 MCPA
93652 MCPP
100027 4-Nitrophenol
87865 Pentachlorophenol (PCP)
1918021 Picloram
93765 2,4,5-T
93721 2,4,5-TP
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